Zn ( II ) - 113 Cd ( II ) and Zn ( I 1 ) - Mg ( I 1 ) Hybrids of Alkaline Phosphatase 31
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چکیده
Methods have been developed for the addition of different metal ion species to the three distinct pairs of metal sites (A, B, and C) found in the dimer of apoalkaline phosphatase. This allows the preparation of hybrid alkaline phosphatases in which A and B sites of each monomer contain two different species of metal ion or the A and B sites of one monomer contain the same species of metal ion, while the adjacent monomer contains a second species. The following hybrids have been characterized in detail: (zn(II)Acd(II)& alkaline phosphatase, (Zn(II)AMg(II)B)a lkaline phosphatase, (Cd (11) AZn (11) B ) Z alkaline phosphatase, and
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